RESEARCH TECHNICIAN II

Occupational Summary

Perform a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of Duke University research operations.

The applicant will join the newly established Roger Lab at Duke University. The research explores the characterization of respiratory deficits and neuromuscular weakness using clinically relevant techniques and develop novel, translational therapies to treat these pathologies. The laboratory uses a variety of techniques including molecular biology (cloning, PCR, mammalian cell culture, qPCR, and proteomics), genetics (recombinant viral vectors – AAV, mouse breeding), imaging (histology, light microscopy, confocal microscopy), and strength analyses (whole body plethysmography to evaluate respiration, limb function tests). The Roger Lab is dedicated to inclusive mentorship and training. If you have a bachelor’s degree in any area of the natural sciences and you want to perform research in a supportive and dynamic environment, please apply.

Work Performed

• Manage mouse colony including breeding, weaning, transfers, transport, and supportive care.

• Perform and compile data related to molecular analyses including PCR, RT-qPCR, and western blots to evaluate mouse tissues.

• Perform and compile data related to whole-body plethysmography (respiratory analysis) and limb function tests on mice.

• For some of our experiments, a group of mice will need to be assessed each day, including weekends. This responsibility will be shared between you and other members of the lab.

• Meet or communicate with supervisors and PI on a regular basis to plan tasks, troubleshoot problems, and discuss results.

• Maintain accurate and thorough records in a timely manner.

• Manage laboratory equipment and inventory.

• Carry out routine laboratory duties including routine maintenance, sterilizing glassware and disposables, and disposal of laboratory waste.

• Participate in weekly lab meetings to discuss results or literature.

• Perform other related duties incidental to the work described herein.

• The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.

Required Qualifications at this Level

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.